Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane.
نویسندگان
چکیده
The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, demonstrating the feasibility of using aptamer probes to study diffusion within lipid membranes.
منابع مشابه
A Tethered Bilayer Assembled on Top of Immobilized Calmodulin to Mimic Cellular Compartmentalization
BACKGROUND Biomimetic membrane models tethered on solid supports are important tools for membrane protein biochemistry and biotechnology. The supported membrane systems described up to now are composed of a lipid bilayer tethered or not to a surface separating two compartments: a "trans" side, one to a few nanometer thick, located between the supporting surface and the membrane; and a "cis" sid...
متن کاملBiomolecular Sensing with Colorimetric Vesicles
This chapter summarizes recent studies employing colorimetric vesicle-based systems for biomolecular sensing. Vesicular aggregates exhibit an important advantage as a biological sensing platform in that they mimic the cell membrane—the site of molecular docking, ligand–receptor binding, and other important processes that can be exploited as a means of signal generation. Particularly attractive ...
متن کاملAn evidence for a potassium channel in endoplasmic reticulum based on single channel recording in bilayer lipid membrane
Introduction Numerous studies have demonstrated the presence of potassium selective channels in membranes internal organelles. These channels are essential to a large variety of cellular processes including intracellular 2+ a signaling, protein recycling, charge neutralization and cell protection. In contrast to the sarcoplasmic reticulum + here potassium channels have been clearly ...
متن کاملThe role of spacers on the self-assembly of DNA aptamer-amphiphiles into micelles and nanotapes.
The self-assembly of single-stranded DNA (ssDNA) aptamer-amphiphiles was influenced by the choice of spacer used to link the hydrophobic tail and aptamer headgroup. Aptamer-amphiphiles without spacers or with hydrophilic spacers formed globular micelles while amphiphiles with hydrophobic spacers formed bilayer nanotapes, which are the first such structures formed by DNA-amphiphiles.
متن کاملMolecular Dynamics of Membrane-Spanning DNA Channels: Conductance Mechanism, Electro-Osmotic Transport, and Mechanical Gating.
DNA self-assembly has emerged as a new paradigm for design of biomimetic membrane channels. Several experimental groups have already demonstrated assembly and insertion of DNA channels into lipid bilayer membranes; however, the structure of the channels and their conductance mechanism have remained undetermined. Here, we report the results of molecular dynamics simulations that characterized th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 48 31 شماره
صفحات -
تاریخ انتشار 2012